Meniscal Mineralization in Domestic Cats

Objective: To (1) determine prevalence of radiographically detectable meniscal mineralization in domestic cats and (2) to evaluate the association between meniscal mineralization and degenerative joint disease (DJD). Study Design: Prospective study. Animals: Client‐owned cats (n=100) and 30 feline cadavers. Methods: Randomly selected client‐owned cats were used to determine the prevalence of meniscal mineralization. Stifles from feline cadavers were used to evaluate the relationship between meniscal mineralization (using high‐resolution X‐ray), radiographic DJD, and cartilage damage. Menisci were evaluated histologically. Results: Forty‐six percent of the client‐owned cats had meniscal mineralization detected in 1 or both stifles. Pain scores were not significantly different between stifles with meniscal mineralization and those with no radiographic pathology (P=.38). Thirty‐four of 57 cadaver stifles had meniscal mineralization, which was always located in the cranial horn of the medial meniscus. Percentage mineralization of the menisci was significantly correlated with the cartilage damage score of the medial femoral (r²=0.6; P<.0001) and tibial (r²=0.5; P<.0001) condyles as well as with the total joint cartilage damage (r²=0.36; P<.0001) score and DJD score (r²=0.8; P<.0001). Conclusion: Meniscal mineralization is a common condition in domestic cats and seems to indicate medial compartment DJD. Clinical Relevance: Clinical significance of meniscal mineralization is uncertain. Further work is needed to determine if the meniscal mineralization is a cause, or a consequence of joint degeneration.     

山羊卵母细胞体外成熟因素的研究

建立和优化山羊卵母细胞体外成熟的培养体系,对山羊卵母细胞的采集方法、割卵液种类、培养液添加血清种类等因素进行了研究.结果显示切剖法与抽吸法对山羊卵母细胞的成熟有显著差异(P<0.05);割卵液DMEM/F-12与PBS+BSA对山羊卵母细胞的成熟有极显著差异(P<0.01);添加EGS(自然发情)、EGS(PG诱导发情)、FBS对山羊卵母细胞的成熟有极显著差异(P<0.01).结论:切剖法能更好地支持卵母细胞的成熟;DMEM/F-12作为割卵液,能显著提高卵母细胞的成熟率;自然发情的山羊血清对卵母细胞的成熟效果最好,同种血清对卵母细胞的成熟效果要好于异种血清.     

鱼油乳状液的制备及其流变特性研究

采用食品级Span系列乳化剂和羧甲基纤维素钠(CMC)水溶液经超声乳化制备鱼油乳状液,比较了乳化剂、CMC浓度、油水比、超声乳化次数等工艺参数对鱼油乳液稳定性的影响,确定了最佳工艺参数:鱼油和Span80(质量比9:1)为油相,0.5%CMC溶液为水相,油水比1:2,超声乳化10次(每次超声5 s、间歇5 s).上述条件制备的乳液,静置24 h和3000 r·min-1离心5 min平均相对体积分别为99.78%和98.32%.显微镜下观察形态均一,乳液平均粒径约为5μm.稳态扫描结果表明鱼油乳液呈现典型的剪切变稀流变行为,频率扫描结果表明鱼油乳液具有一定的粘弹性.     

亚洲玉米螟幼虫体内储存蛋白cDNA片段克隆与序列分析

为研究亚洲玉米螟Ostrinia furnacalis(Guen e)幼虫体内储存蛋白(SP)表达调控的分子机制,根据不同昆虫储存蛋白基因序列的保守区域,设计合成简并引物,采用RT-PCR技术从亚洲玉米螟5龄幼虫中扩增出一段cDNA片段,大小为892bp,编码297个氨基酸,预测分子量为35.7kD,理论等电点pI值为8.73。该基因序列中含有保守的幼虫储存蛋白(LSP)两个特征基序YFTEDIDLNTYYYYFHVDYP和TALRDPVFC。Blast P分析结果表明:该片段推导的氨基酸序列与斜纹夜蛾Spodoptera litura适量甲硫氨酸SP-1的一致性最高,为77%;与小菜蛾Plutella xylostella富含甲硫氨酸SP-1和SP-2、印度谷螟Plodia interpunctellaSP、美国白蛾Hyphantria cuneaSP-2和粉茎螟Sesamia nonagrioides适量甲硫氨酸SP氨基酸序列的一致性分别为74%、74%、72%、71%和65%。构建系统发育树分析了9种鳞翅目昆虫储存蛋白进化关系,结果显示:亚洲玉米螟cDNA片段氨基酸序列与小菜蛾富含甲硫氨酸SP-1的亲缘关系最近,与粉茎螟适量甲硫氨酸SP亲缘关系最远。     

白朗县白雪试验站春油菜品比试验

参试材料川油13号生育期、分枝性状等其它性状上综合,均优于对照,是试验最优品种(系)。年河主要农区,适合选育生育期为110～130天的甘蓝型春油菜品种。建议油菜育种,能够提供生育期较为早熟的甘蓝型品种,提供给生产上使用。     

西藏牦牛遗传资源保护与利用

牦牛是我国丰富多样的畜种资源中重要的畜种之一,在遗传上是一个极为宝贵的基因库。本文阐述了西藏牦牛遗传资源的保种方法和利用途径,有助于西藏畜牧业的可持续发展。     

Agarase: Review of Major Sources,Categories, Purification Method,Enzyme Characteristics and Applications

Agarases are the enzymes which catalyze the hydrolysis of agar. They are classified into α-agarase (E.C. 3.2.1.158) and β-agarase (E.C. 3.2.1.81) according to the cleavage pattern. Several agarases have been isolated from different genera of bacteria found in seawater and marine sediments, as well as engineered microorganisms. Agarases have wide applications in food industry, cosmetics, and medical fields because they produce oligosaccharides with remarkable activities. They are also used as a tool enzyme for biological, physiological, and cytological studies. The paper reviews the category, source, purification method, major characteristics, and application fields of these native and gene cloned agarases in the past, present, and future.     

Three AFLP markers tightly linked to the genic male sterility <Emphasis Type="Italic">ms</Emphasis><Subscript><Emphasis Type="Italic">3</Emphasis></Subscript> gene in chili pepper (<Emphasis Type="Italic">Capsicum annuum</Emphasis> L.) and conversion to a CAPS marker

Genic male sterility (GMS) has long been used as a tool for hybrid seed production in chili pepper (Capsicum annuum L.). We developed DNA markers linked to the GMS ms ₃ gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) techniques. The segregating population was subjected to BSA-AFLP with 512 primer combinations. Three AFLP markers (Eagg/Mccc₂₇₆, Eagc/Mctt₁₇₈, and Ecag/Mtgc₂₀₄) were identified as tightly linked to the ms ₃ locus. Among them, we converted the AFLP marker Ecag/Mtgc₂₀₄ to the cleavage amplified polymorphic sequence (CAPS) marker, named GMS3-CAPS, based on sequencing analysis of internal and flanking regions for the markers between male-fertile and sterile plants. This marker will be useful for pepper breeding using the GMS system.     

露地番茄品种比较试验

引进12个番茄品种并对其丰产性、商品性和适口性进行比较试验。试验结果表明：在参试的12个品种中,综合性状最好的是‘毛粉812’,其次是‘春秋粉宝’,表现丰产、整齐度高、果形端正等,适合本地区栽植,有很好的推广价值,应加大推广力度;‘新选906’为自封顶类型,商品果小,产量低且综合性状差,不适合在春季露地栽培。